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antibody ib4-488 thermo fisher cat#: i21411  (Thermo Fisher)
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Antibody Ib4 488 Thermo Fisher Cat#: I21411, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Antibody Ib4 488, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 2 Db/db mice + 1% salt show microvascular dysfunction before the onset of diastolic dysfunction. (A, B) Cardiac capillary density measured as the number of <t>IB4</t> + microvessels per area in db/+ and db/db + 1% salt mice at 2, 4, 6 and 8 weeks with representative images (n = 5–8/group). (C) Number of pericytes was determined by counting the cells that were double positive for NG2 and PDGFRb marker, and co-localised with IB4 + microvessels. (D) Pericyte coverage, assessed by the ratio of IB4 + microvessels covered by NG2/PDGFRb double positive cells over total IB4 + microvessels. (E, F) Active vessel regression, assessed by the ratio of Coll IV + IB4- microvessels over total IB4 + microvessels with representative images at 4 and 6 weeks (n = 5–8/ group). White arrowheads indicate empty collagen sleeves. Scalebars are 6 μm. Values are presented as mean ± SEM. Two-way ANOVA followed by Sidak’s multiple comparisons test with *p < 0.05, **p < 0.01, and ***p < 0.001. IB4, isolectin B4; NG2, neural glial antigen 2; NS, non-significant; PDGFRb, platelet derived growth factor receptor beta; Coll IV, collagen IV. At 2 weeks: n = 8 F (1 db/+, 7 db/db) and 5 M (4db/+, 1 db/db); at 4 weeks: n = 7 F (4 db/+, 3 db/ db) and 8 M (4 db/+, 4db/db); at 6 weeks: n = 7 F (3 db/+, 4 db/db) and 6 M (3 db/+, 3 db/db), at 8 weeks: 4 F (2 db/+, 2db/db) and 6 M (3 db/+, 3 db/db)
Ib4, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 2 Db/db mice + 1% salt show microvascular dysfunction before the onset of diastolic dysfunction. (A, B) Cardiac capillary density measured as the number of <t>IB4</t> + microvessels per area in db/+ and db/db + 1% salt mice at 2, 4, 6 and 8 weeks with representative images (n = 5–8/group). (C) Number of pericytes was determined by counting the cells that were double positive for NG2 and PDGFRb marker, and co-localised with IB4 + microvessels. (D) Pericyte coverage, assessed by the ratio of IB4 + microvessels covered by NG2/PDGFRb double positive cells over total IB4 + microvessels. (E, F) Active vessel regression, assessed by the ratio of Coll IV + IB4- microvessels over total IB4 + microvessels with representative images at 4 and 6 weeks (n = 5–8/ group). White arrowheads indicate empty collagen sleeves. Scalebars are 6 μm. Values are presented as mean ± SEM. Two-way ANOVA followed by Sidak’s multiple comparisons test with *p < 0.05, **p < 0.01, and ***p < 0.001. IB4, isolectin B4; NG2, neural glial antigen 2; NS, non-significant; PDGFRb, platelet derived growth factor receptor beta; Coll IV, collagen IV. At 2 weeks: n = 8 F (1 db/+, 7 db/db) and 5 M (4db/+, 1 db/db); at 4 weeks: n = 7 F (4 db/+, 3 db/ db) and 8 M (4 db/+, 4db/db); at 6 weeks: n = 7 F (3 db/+, 4 db/db) and 6 M (3 db/+, 3 db/db), at 8 weeks: 4 F (2 db/+, 2db/db) and 6 M (3 db/+, 3 db/db)
Isolectin Gs Ib4 488 Ib4 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 2 Db/db mice + 1% salt show microvascular dysfunction before the onset of diastolic dysfunction. (A, B) Cardiac capillary density measured as the number of <t>IB4</t> + microvessels per area in db/+ and db/db + 1% salt mice at 2, 4, 6 and 8 weeks with representative images (n = 5–8/group). (C) Number of pericytes was determined by counting the cells that were double positive for NG2 and PDGFRb marker, and co-localised with IB4 + microvessels. (D) Pericyte coverage, assessed by the ratio of IB4 + microvessels covered by NG2/PDGFRb double positive cells over total IB4 + microvessels. (E, F) Active vessel regression, assessed by the ratio of Coll IV + IB4- microvessels over total IB4 + microvessels with representative images at 4 and 6 weeks (n = 5–8/ group). White arrowheads indicate empty collagen sleeves. Scalebars are 6 μm. Values are presented as mean ± SEM. Two-way ANOVA followed by Sidak’s multiple comparisons test with *p < 0.05, **p < 0.01, and ***p < 0.001. IB4, isolectin B4; NG2, neural glial antigen 2; NS, non-significant; PDGFRb, platelet derived growth factor receptor beta; Coll IV, collagen IV. At 2 weeks: n = 8 F (1 db/+, 7 db/db) and 5 M (4db/+, 1 db/db); at 4 weeks: n = 7 F (4 db/+, 3 db/ db) and 8 M (4 db/+, 4db/db); at 6 weeks: n = 7 F (3 db/+, 4 db/db) and 6 M (3 db/+, 3 db/db), at 8 weeks: 4 F (2 db/+, 2db/db) and 6 M (3 db/+, 3 db/db)
Isolectin Gs Ib 4 488 Ib4 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fitc-conjugated ib4 l2895 antibody  (Millipore)
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Fig. 2 Db/db mice + 1% salt show microvascular dysfunction before the onset of diastolic dysfunction. (A, B) Cardiac capillary density measured as the number of <t>IB4</t> + microvessels per area in db/+ and db/db + 1% salt mice at 2, 4, 6 and 8 weeks with representative images (n = 5–8/group). (C) Number of pericytes was determined by counting the cells that were double positive for NG2 and PDGFRb marker, and co-localised with IB4 + microvessels. (D) Pericyte coverage, assessed by the ratio of IB4 + microvessels covered by NG2/PDGFRb double positive cells over total IB4 + microvessels. (E, F) Active vessel regression, assessed by the ratio of Coll IV + IB4- microvessels over total IB4 + microvessels with representative images at 4 and 6 weeks (n = 5–8/ group). White arrowheads indicate empty collagen sleeves. Scalebars are 6 μm. Values are presented as mean ± SEM. Two-way ANOVA followed by Sidak’s multiple comparisons test with *p < 0.05, **p < 0.01, and ***p < 0.001. IB4, isolectin B4; NG2, neural glial antigen 2; NS, non-significant; PDGFRb, platelet derived growth factor receptor beta; Coll IV, collagen IV. At 2 weeks: n = 8 F (1 db/+, 7 db/db) and 5 M (4db/+, 1 db/db); at 4 weeks: n = 7 F (4 db/+, 3 db/ db) and 8 M (4 db/+, 4db/db); at 6 weeks: n = 7 F (3 db/+, 4 db/db) and 6 M (3 db/+, 3 db/db), at 8 weeks: 4 F (2 db/+, 2db/db) and 6 M (3 db/+, 3 db/db)
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Fig. 2 Db/db mice + 1% salt show microvascular dysfunction before the onset of diastolic dysfunction. (A, B) Cardiac capillary density measured as the number of IB4 + microvessels per area in db/+ and db/db + 1% salt mice at 2, 4, 6 and 8 weeks with representative images (n = 5–8/group). (C) Number of pericytes was determined by counting the cells that were double positive for NG2 and PDGFRb marker, and co-localised with IB4 + microvessels. (D) Pericyte coverage, assessed by the ratio of IB4 + microvessels covered by NG2/PDGFRb double positive cells over total IB4 + microvessels. (E, F) Active vessel regression, assessed by the ratio of Coll IV + IB4- microvessels over total IB4 + microvessels with representative images at 4 and 6 weeks (n = 5–8/ group). White arrowheads indicate empty collagen sleeves. Scalebars are 6 μm. Values are presented as mean ± SEM. Two-way ANOVA followed by Sidak’s multiple comparisons test with *p < 0.05, **p < 0.01, and ***p < 0.001. IB4, isolectin B4; NG2, neural glial antigen 2; NS, non-significant; PDGFRb, platelet derived growth factor receptor beta; Coll IV, collagen IV. At 2 weeks: n = 8 F (1 db/+, 7 db/db) and 5 M (4db/+, 1 db/db); at 4 weeks: n = 7 F (4 db/+, 3 db/ db) and 8 M (4 db/+, 4db/db); at 6 weeks: n = 7 F (3 db/+, 4 db/db) and 6 M (3 db/+, 3 db/db), at 8 weeks: 4 F (2 db/+, 2db/db) and 6 M (3 db/+, 3 db/db)

Journal: Cardiovascular diabetology

Article Title: Mural cell dysfunction contributes to diastolic heart failure by promoting endothelial dysfunction and vessel remodelling.

doi: 10.1186/s12933-025-02623-w

Figure Lengend Snippet: Fig. 2 Db/db mice + 1% salt show microvascular dysfunction before the onset of diastolic dysfunction. (A, B) Cardiac capillary density measured as the number of IB4 + microvessels per area in db/+ and db/db + 1% salt mice at 2, 4, 6 and 8 weeks with representative images (n = 5–8/group). (C) Number of pericytes was determined by counting the cells that were double positive for NG2 and PDGFRb marker, and co-localised with IB4 + microvessels. (D) Pericyte coverage, assessed by the ratio of IB4 + microvessels covered by NG2/PDGFRb double positive cells over total IB4 + microvessels. (E, F) Active vessel regression, assessed by the ratio of Coll IV + IB4- microvessels over total IB4 + microvessels with representative images at 4 and 6 weeks (n = 5–8/ group). White arrowheads indicate empty collagen sleeves. Scalebars are 6 μm. Values are presented as mean ± SEM. Two-way ANOVA followed by Sidak’s multiple comparisons test with *p < 0.05, **p < 0.01, and ***p < 0.001. IB4, isolectin B4; NG2, neural glial antigen 2; NS, non-significant; PDGFRb, platelet derived growth factor receptor beta; Coll IV, collagen IV. At 2 weeks: n = 8 F (1 db/+, 7 db/db) and 5 M (4db/+, 1 db/db); at 4 weeks: n = 7 F (4 db/+, 3 db/ db) and 8 M (4 db/+, 4db/db); at 6 weeks: n = 7 F (3 db/+, 4 db/db) and 6 M (3 db/+, 3 db/db), at 8 weeks: 4 F (2 db/+, 2db/db) and 6 M (3 db/+, 3 db/db)

Article Snippet: Paraffin sections were stained with IB4 and rabbit anti-p21 antibody (Novus, NB100-1941, 1/50).

Techniques: Marker, Derivative Assay